Evaluation of the DAS-ELISA as a Detection Method for Rice stripe virus from Its Vector Insect, Small Brown Planthopper, Laodelphax striatellus
نویسندگان
چکیده
منابع مشابه
Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus
Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcrip...
متن کاملTransmission of Rice stripe virus acquired from frozen infected leaves by the small brown planthopper (Laodelphax striatellus Fallen).
Rice stripe disease, caused by Rice stripe virus (RSV), is one of the most serious rice diseases in temperate and subtropical regions of the world. Since RSV is not transmissible mechanically, an insect transmission test was the original basis for identification of the viral population and cultivar resistance. A simple, rapid and reliable method is described by which virus-free small brown plan...
متن کاملGenome sequence of the small brown planthopper, Laodelphax striatellus
Background Laodelphax striatellus Fallén (Hemiptera: Delphacidae) is one of the most destructive rice pests. L. striatellus is different from 2 other rice planthoppers with a released genome sequence, Sogatella furcifera and Nilaparvata lugens, in many biological characteristics, such as host range, dispersal capacity, and vectoring plant viruses. Deciphering the genome of L. striatellus will f...
متن کاملProbing stimulants from the rice plant towards the smaller brown planthopper, Laodelphax striatellus (FALLEN) (Homoptera: Delphacidae).
When adult males of the smaller brown planthopper, Laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. Further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. When the butanol fr. was charged on an ODS open column and el...
متن کاملDevelopment and characterization of nine polymorphic microsatellites for the small brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae).
Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)(6)(AC)(5) and (AG)(6)(AC)(5) compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated (Jiangsu, Shandong and Zhejiang). The observe...
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ژورنال
عنوان ژورنال: Japanese Journal of Applied Entomology and Zoology
سال: 2013
ISSN: 0021-4914,1347-6068
DOI: 10.1303/jjaez.2013.113